Fiberoptic Microneedle Device for Nanoparticle-Enhanced Photothermal Therapy of Aggressive, Infiltrative Bladder Cancer

Sponsor: NIH: NCI: R21 CA156078
Performance Period: 1/1/11-12/31/12
PI: Chris Rylander, co-PIs: M. Nichole Rylander, John Robertson, and David Grant

The goal of this project is to develop a more effective and selective treatment for invasive bladder cancer in which the bladder integrity and that of accessory organs can be preserved, while ensuring adequate tumor regression in the muscle/serosal layers. To accomplish this goal, a fiberoptic microneedle device (FMD) will be developed and utilized as outlined in the Specific Aims:

Specific Aim 1: Demonstrate the capability of individual FMD microneedles for targeted penetration into selected bladder depths and nanoparticle and photothermal delivery in excised urinary bladders from normal rats, dogs, and pigs.

Specific Aim 2: Integrate multifunctional optical and nanoparticle delivery capabilities of FMD within a clinical cystoscope and evaluate on excised urinary bladders.

Specific Aim 3: Evaluate the capability of the FMD/cystoscope for effective nanoparticle-enhanced photothermal therapy in rodent and canine models of invasive bladder cancer.

Figure 1: Transmission electron microscope image of single-walled carbon nanohorns.Figure 2: Co-delivery of nanohorns and laser light in an ex vivobladder.

Left: Transmission electron microscope image of single-walled carbon nanohorns.

Right: Co-delivery of nanohorns and laser light in an ex vivobladder.